CD33 Rabbit Polyclonal Antibody

SKU: orb2563093

Description

CD33 Rabbit Polyclonal Antibody

Research Area

Human Lineage-negative, Innate Immunity, Myeloid Cells, Stem Cell Research

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human CD33 protein (18-259/364aa)
• Target: CD33
• Molecular Weight: 67 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor.

20 ng rhCD33-His protein per lane probed with CD33 polyclonal antibody respectively, unconjugated (orb2563093) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

The THP-1 (H) cells incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD33 antibody (orb2563093): 1 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 µg/Test. Isotype Control (orange): Rabbit IgG (orb570250). Blank control (black): PBS. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

CD33

UniProt

P20138