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CD33 Rabbit Polyclonal Antibody
SKU: orb10316
Featured
Description
Research Area
Human Lineage-negative, Innate Immunity, Myeloid Cells, Stem Cell Research
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Mouse |
| Predicted Reactivity | Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD33 (101-200/334aa) |
| Target | CD33 |
| Molecular Weight | 67 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor.
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Sample: Lane 1: Bone (Mouse) Lysate at 40 ug, Lane 2: Lymph node (Mouse) Lysate at 40 ug, Primary: Anti-CD33 (orb10316) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40/37 kD, Observed band size: 65 kD.
Quick Database Links
Gene Symbol
CD33
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Simona Panzacchi # 1, Eva Tibaldi # 1, Luana De Angelis 1, Laura Falcioni 1, Rita Giovannini 1, Federica Gnudi 1, Martina Iuliani 1, Marco Manservigi 1, Fabiana Manservisi 1, Isabella Manzoli 1, Ilaria Menghetti 1, Rita Montella 1, Roberta Noferini 1, Daria Sgargi 1, Valentina Strollo 1, Francesca Truzzi 1 2, Michael N Antoniou 3, Jia Chen 4, Giovanni Dinelli 2, Stefano Lorenzetti 5, Alberto Mantovani 6, Robin Mesnage 3, Melissa J Perry 7, Andrea Vornoli 8, Philip J Landrigan # 9 10, Fiorella Belpoggi # 1 11, Daniele Mandrioli Carcinogenic effects of long-term exposure from prenatal life to glyphosate and glyphosate-based herbicides in Sprague-Dawley rats Environ Health, (2025)
Applications
IHC
Reactivity
Rat
CD33 Rabbit Polyclonal Antibody (orb10316)
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