CD11c Antibody

SKU: orb749365

Description

CD11c, also known as Integrin alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute non-lymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Functional studies (order BSA/sodium azide-free format)
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD11c antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: HC1/1
• Immunogen: Human monocytic U937 phorbol ester-treated cells were used as the immunogen for this CD11c antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

FACS staining of human PBMCs using CD11c antibody (HC1/1).