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CCL3 Recombinant Protein
Description
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is 3-10 ng/mL as determined by the ability of Recombinant CCL3 to chemoattract human CCR5 transfected BaF3 mouse proB cells. |
| Target | CCL3 |
| Tag | Tag Free |
| Molecular Weight | 7.5 kD |
| Protein Sequence | Ser24-Ala92 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20˚°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7˚°C for 2-7 days.Aliquots of reconstituted samples are stable at -20˚°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Murine Macrophage Inflammatory Protein-1 alpha/CCL3 [orb1906025]
> 98 % by SDS-PAGE and HPLC analyses.
Approximately 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Escherichia coli
10 μg, 100 μg, 500 μgRecombinant Rat Macrophage Inflammatory Protein-1 alpha/CCL3 [orb1905995]
> 98 % by SDS-PAGE and HPLC analyses.
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Escherichia coli
5 μg, 500 μg, 100 μgHuman CCL3 Protein [orb1471711]
Greater than 95% as determined by reducing SDS-PAGE.
7.5 KDa
Mammalian
10 μg, 50 μgHuman CCL3 Protein, hFc Tag [orb1826856]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide. The apparent molecular mass of CCL3-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg

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Protocol Information
CCL3 Recombinant Protein (orb1229815)
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