CCG-100602

SKU: orb1219035

Description

CCG-100602 inhibits RhoA/C-mediated, SRF-driven luciferase expression in PC-3 prostate cancer cells (IC50 :9.8 μM).

Key Properties

• CAS Number: 1207113-88-9
• MW: 478.82
• Purity: >98% (HPLC)
• Formula: C₂₁H₁₇ClF₆N₂O₂
• SMILES: ClC1=CC=C(NC(C2CN(C(C3=CC(C(F)(F)F)=CC(C(F)(F)F)=C3)=O)CCC2)=O)C=C1
• Target: Rho
• Solubility: DMSO:100 mg/mL (208.85 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Animal model: Adult (4 month-old) male spontaneously hypertensive rats (SHR) and normotensive control Wistar-Kyoto (WKY) rats. Dosage: 7.5 mg/kg/day. Administration: Continuously administered for 2 weeks by osmotic minipumps. Result: Abrogated the increase of aortic stiffness represented by reduced arterial compliance and strain.
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• In Vitro:
  Cell Viability Assay Cell line: Human adipose stem cell (hASC). Concentration: 3, 8, 15, or 30 μM. Incubation time: 7 days. Result: The number of adherent cells decreased as a response to increasing inhibitor amount. The effect was also dependent on the culture media because the osteogenic medium condition supported the viability over basic culture medium and adipogenic medium conditions. RT-PCR Cell line: Human intestinal myofibroblasts (HIMFs). Concentration: 5, 10, 20, and 40 μM. Incubation time: 30 min prior to the addition of TGF-β1 (5 ng/mL) for 24 hours. Result: Diminished the TGF-β1-induced increase in COL1A1, FN1, and ACTA2 transcription in a dose-dependent manner. Reduced the TGF-β1-induced increase in MRTFA and SRF mRNA expression in the HIMFs in a dose-dependent manner. Western blot analysis. Cell line: Human intestinal myofibroblasts (HIMFs). Concentration: 5, 10, 20, and 40 μM. Incubation time: 30 min prior to the addition of TGF-β1 (5 ng/mL) for 48 hours. Result: The protein expression levels of the ECM and α-SMA in TGF-β1-stimulated cells are significantly reduced.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Rho