CAY10404

SKU: orb1298739

Description

CAY10404 is a potent and selective dual COX-1/COX-2 inhibitor that also suppresses PKB/Akt and MAPK signaling. This small molecule induces apoptosis in NSC-LC cells in vitro, supporting its research use in studying inflammation, pain, and cancer pathways.

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction

Key Properties

• CAS Number: 340267-36-9
• MW: 367.34
• Purity: 99.85% (May vary between batches)
• Formula: C₁₇H₁₂F₃NO₃S
• SMILES: CS(=O)(=O)c1ccc(cc1)-c1noc(c1-c1ccccc1)C(F)(F)F
• Target: Akt|||COX|||Apoptosis
• Solubility: DMSO:10 mg/mL (27.22 mM);10% DMSO+90% Corn Oil:1 mg/mL (2.72 mM)

Bioactivity

• Target IC50:
  COX-1:>500 μM|COX-2:1 nM
• In Vitro:
  Treatment with CAY10404 in the range of 10-100 microM caused dose-dependent growth inhibition, with an average 50% inhibitory concentration (IC(50)) of 60-100 micromol/L, depending on the cell line. Western blot analysis of CAY10404-treated cells showed cleavage of poly (ADP-ribose) polymerase (PARP) and procaspase-3, signifying caspase activity and apoptotic cell death. CAY10404 treatment inhibited the phosphorylation of Akt, glycogen synthase kinase-3beta and extracellular signal-regulated kinases 1/2 in H460 and H358 cells
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• Cell Research:
  To measure the effects of CAY10404 on proliferation of NSCLC cells, 3 x 10(3) cells/well were plated in 96-well plates and allowed to adhere overnight at 37 degrees C. After treatment with CAY10404 for 3 days, cell proliferation was measured by the 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In the H460 NSCLC cells, evidence of apoptosis was sought using the terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) assay and western blot analysis.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, anti-inflammatory, anti-cancer, analgesic, cancer, Bcl-XL, Bcl-2, 3-(4-METHYLSULPHONYLPHENYL)-4-PHENYL-5-T, Akt, COX, cell, CAY 10404, CAY10404, CAY-10404, COX-1, COX-2, Cyclooxygenase, cyclooxygenase-2, H-358, H-460, H-1703, Inhibitor, MAPK, inhibit, Non-small, lung, pAkt, NSCLC, PKB, Protein kinase B, pGSK-3β

Compound Identifiers

PubChem CID

10429020