CAV1 Antibody

SKU: orb345781

Description

Caveolin 1 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1: 10,000, WB: 1ug/ml
• Reactivity: Human
• Application Notes:
  Anti-Caveolin1 antibody has been tested in western blot and is useful for ELISA and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~21kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Caveolin 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide at the N-terminus of human Caveolin-1 protein.
• Target: CAV1
• Purity: Anti-Caveolin 1 was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with sheep, primate, bat, rat, rabbit, mouse, elephant, horse, human, cat, armadillo, and hedgehog based on 100% sequence homology. Cross-reactivity with Caveolin 1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 30% Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.68 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Caveolin-1 Antibody, CAV1, CAV, CAV 1, rabbit anti-Caveolin 1 Antibody

Western Blot of Rabbit Anti-Caveolin 1 Antibody. Lane 1: PG13 Whole Cell Lysate - Reduced [5 µg]. Lane 2: PG13 Whole Cell Lysate - Reduced [1 µg]. Lane 3: Opal Prestained MW Marker Lane 4: PG13 Cytosolic Extract - Reduced [1 µg]. Lane 5: PG13 Cytosolic Extract - Reduced [5 µg]. Primary Antibody: Anti-Caveolin-1 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase Conjugated (p/n orb347654) at 1:70000 for 1 hour at RT. Block: BlockOut Blocking Buffer (p/n orb348644) overnight at 2-8°C. Predicted MW: ~21kDa. Exposure: 5 sec.

Quick Database Links

Gene Symbol

CAV1

UniProt

Q03135