Cattle Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

• Catalog Number: orb779316
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 1(TGFb1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1(TGFb1).
• Reactivity: Bovine
• Concentration: 1000 pg/mL
• UniProt ID: P18341
• Tested applications: ELISA
• Application notes: standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.8 pg/mL
• Sample Types: serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
• Alternative names: TGF Beta 1,TGF-B1 ELISA Kit, CED ELISA Kit, DPD1 E
• Research Area: Cancer Biology, Immunology & Inflammation, Infecti
• Note: For research use only
• Expiration Date: Please enquire.