Cattle Ornithine Carbamoyl Transferase (OCT) ELISA Kit

SKU: orb781776

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Carbamoyl Transferase(OCT).

Research Area

Metabolism Research, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle OCT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle OCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle OCT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle OCT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Bovine
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 6.25-400 ng/mL
• Sensitivity: 2.47 ng/mL
• Concentration: 400 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

OCTD ELISA Kit, OTCase ELISA Kit, Ornithine Transcarbamylase ELISA Kit, Ornithine Carbamoyltransferase ELISA Kit