Cattle Immunoglobulin E (IgE) ELISA Kit

SKU: orb1736543

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IgE in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Application Notes:
  standard: 4000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IgE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Bovine
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 ng/mL
• Sensitivity: 28.3 ng/mL
• Concentration: 4000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region