Cattle Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit

• Catalog Number: orb779462
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein(a1AGP).
• Reactivity: Bovine
• Concentration: 1000 ng/mL
• UniProt ID: Q3SZR3
• Tested applications: ELISA
• Application notes: standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle a1AGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle a1AGP in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 7 ng/mL
• Sample Types: serum, plasma, urine and other biological fluids
• Alternative names: alpha1-AGP,AGP1 ELISA Kit, a1-AGP ELISA Kit, ORM1
• Research Area: Gastroenterology & Hepatology, Immunology & Inflam
• Note: For research use only