Cattle Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit

SKU: orb779462

Description

The Cattle Alpha-1-Acid Glycoprotein (a1AGP) ELISA Kit uses a sandwich enzyme immunoassay to quantify Alpha-1-Acid Glycoprotein (a1AGP) in bovine serum, plasma, urine and other biological fluids. The kit has a detection range of 15.63-1000 ng/mL and a sensitivity of 7 ng/mL.

Research Area

Gastroenterology & Hepatology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle a1AGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle a1AGP in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Bovine
• Sample Types: serum, plasma, urine and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 7 ng/mL
• Concentration: 1000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

alpha1-AGP,AGP1 ELISA Kit, a1-AGP ELISA Kit, ORM1 ELISA Kit, OMD 1 ELISA Kit, Orosomucoid 1 ELISA Kit

Quick Database Links

UniProt

Q3SZR3