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Catalog Number | orb346567 |
---|---|
Category | Antibodies |
Description | Cat IgG F(ab')2 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Feline |
Isotype | IgG |
Immunogen | Cat IgG F(ab')2 fragment |
Concentration | 10.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgG, Cat IgG F(ab’)2 and Cat Serum. No reaction was observed against Cat IgG F(c). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | None |
Alternative names | rabbit anti-Cat IgG F(ab')2 fragment Antibody, rab Read more... |
Note | For research use only |
Application notes | Anti-Cat IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
TRITC | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin. | |
Cat |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin. | |
Cat |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin. | |
Cat |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin. | |
Cat |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin. | |
Cat |
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