Cat IgG F(ab')2 Antibody

SKU: orb346024

Description

Cat IgG F(ab')2 Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  CAT IgG F(ab')2 fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Cat
• Biological Origin: Cat
• Isotype: IgG F(ab')2
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Cat IgG, anti-Cat IgG F(ab’)2 and anti-Cat Serum. No reaction was observed against anti-Cat IgG F(c) or anti-Pepsin.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cat IgG F(ab')2 fragment, Cat IgG Fab2 fragment

SDS-PAGE Results of Cat F(ab')2 fragment. Lane 1: Cat F(ab')2 Reduced 10 µg. Lane 2: Cat F(ab')2 Reduced 5 µg. Lane 3: Cat F(ab')2 Reduced 1 µg. Lane 4: Opal Prestained Molecular Weight Marker. Lane 5: Cat F(ab')2 Non-Reduced 10 µg. Lane 6: Cat F(ab')2 Non-Reduced 5 µg. Lane 7: Cat F(ab')2 Non-Reduced 1 µg. 4-20% Gel, Coomassie stained.