CAS9 Protein

SKU: orb753020

Description

Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease

Research Area

Molecular Biology

Images & Validation

• Application Notes:
  Enzymatic Activity: 97% of PCR product digestion after 30 minutes at 37°C. Endonuclease Activity: The incubation of 20pmol of CAS9 protein with 1µg pUC19 supercoiled vector at 37°C for 4 hours leads to formation of less than 10% open circular pUC19 form as determined by gel electrophoresis. Non-specific DNAse activity: The incubation of 20pmol of CAS9 protein with 1µg of lambda DNA at 37°C for 4 hours is free of detectable DNA degradation, as visualized on agarose gel

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Key Properties

• Source: Escherichia Coli
• Purity: Greater than 90% as determined by SDS-PAGE.

Storage & Handling

• Storage: Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles
• Form/Appearance: Sterile Filtered colorless solution.
• Buffer/Preservatives: The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.