β-Caryophyllene

SKU: orb1298358

Description

β-Caryophyllene

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 87-44-5
• MW: 204.35
• Purity: 99.11%
• Formula: C₁₅H₂₄
• SMILES: [H][C@]12CC(C)(C)[C@]1([H])CC\C(C)=C/CCC2=C
• Target: Endogenous Metabolite,Cannabinoid Receptor
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (9.79 mM);DMSO:25 mg/mL (122.34 mM);Ethanol:176.67 mg/mL (864.55 mM);H2O:< 0.1 mg/mL (insoluble)

Bioactivity

• In Vivo:
  Durin the examination period, administering varying doses of β-Caryophyllene had no impact on swimming speed. however, oral administration of β-Caryophyllene significantly reduced β-amyloid deposition in transgenic mice in a nearly dose-dependent manner, wit the two highest doses showing comparable efficacy in decreasing β-amyloid accumulation. Notably, Vehicle-treated mice displayed an increased number of activated astroglial cells compared to those treated with any dose of β-Caryophyllene. furthermore , β-Caryophyllene effectively lowere the elevated COX-2 protein levels observed in Vehicle-treated APP/PS1 mice. Additionally, animals receiving β-Caryophyllene treatment demonstrated a higher object recognition index than their Vehicle-treated counterparts, indicating improved memory retention [t(14 = .204, < .05]. There was no significant difference in the total time spent exploring objects betwee the β-Caryophyllene and Vehicle-treated groups durin the test trial (t(14 = .5874, P>0.05). β-Caryophyllene treatment did not significantly affect seizure-induced neurochemical alterations.
• In Vitro:
  β-Caryophyllene shows Selective anti Proliferative effects against three tested cancer cell lines: HCT 116 (colon cancer, IC50 19 μM), PANC-1 (pancreatic cancer, IC50 27 μM), and HT29 (colon cancer, IC50 63 μM), but exhibits moderate or poor cytotoxicity against ME-180, PC3, K562, and MCF-7. It demonstrates higher selectivity for colorectal cancer cells, particularly HCT 116 (S = 7.9), followed by PANC-1 (S = 9.6) and HT 29 (S = ) the apoptotic index for HCT 116 cells after 24 hours of β-Caryophyllene treatment is 6 ± .04. At 10 μM, it causes significant nuclear condensation after 6 hours and exhibits dose- and time-dependent inhibitory effects on HCT 116 cell motility.
• Cell Research:
  Stock solution (10 mM) of β-Caryophyllene is prepared using DMSO. Further, various concentration (3 to 100 μM) of β-Caryophyllene are prepared by serially dilutin the stock with respective culture medium.Panel of human cancer cells such as, pancreatic (PANC-1), colorectal (HCT-116 and HT-29), invasive squamous cell carcinoma (ME-180), leukemia (K562), hormone sensitive and invasive breast cancer cell line (MCF-7), and prostatic (PC3) adenocarcinoma cell lines are used. Cells are incubated in a humidified CO2 incubator at 37℃upplied with 5% CO2. inhibitory effect of β-Caryophyllene on proliferation o the cell lines is tested usin the MTT assay the selectivity index (SI) fo the cytotoxicity of β-Caryophyllene is calculated usin the ratio of IC50 of β-Caryophyllene on a normal cell line (NIH-3T3) to the IC50 of β-Caryophyllene on cancer cell lines. They are for reference only.
• Animal Research:
  β-Caryophyllene is initially dissolved in dimethyl sulfoxide and further diluted (2%) in sterile phosphate-buffered saline (PBS) with 10% Cremophor EL (poluoxyl-35 hydrogenated castor oil) Male double transgenic APP/PS1 mice and wild-type littermates are used the mice are group housed (3 to 5 animals/cage) with a 12:12 hour light/dark cycle and ad libitum access to food and water. In this experiment, animals are orally treated by gavage with 16, 48, or 144 mg/kg of β-Caryophyllene every morning for 10 weeks starting a the age of 7 months. All Vehicle solutions are used fo the respective control animal treatments an the Morris water maze test is performed. They are for reference only.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

beta-Caryophyllene, CB2, CB, (−)-trans-Caryophyllene, (−)-b-caryophyllene, (−)-beta-caryophyllene, (-)-(E)-Caryophyllene, (−)-β-caryophyllene, CannabinoidReceptor, Cannabinoid Receptor, Caryophyllene, b-Caryophyllene, Inhibitor, EndogenousMetabolite, Endogenous Metabolite, inhibit, β Caryophyllene, βCaryophyllene, β-Caryophyllene

Compound Identifiers

PubChem CID

5281522