Carbonic Anhydrase I antibody (Peroxidase)

• Catalog Number: orb344358
• Category: Antibodies
• Description: Carbonic Anhydrase I antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Carbonic Anhydrase I [Human Erythrocytes ]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P00915
• NCBI: NP_001122301.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat anti-Carbonic Anhydrase I Antibody HRP Conjug
• Note: For research use only
• Application notes: Anti-Carbonic Anhydrase I peroxidase is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers.
• Expiration Date: 12 months from date of receipt.