CA1 Antibody (HRP)

SKU: orb344357

Description

Carbonic Anhydrase I antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Human
• Application Notes:
  Anti-Carbonic Anhydrase I peroxidase is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carbonic Anhydrase I [Human Erythrocytes ]
• Target: CA1
• Purity: Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Carbonic Anhydrase I Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Western Blot of Goat Anti-Carbonic Anhydrase I Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight. Lane 2: Carbonic Anhydrase I [10 µg]. Primary antibody: Anti-Carbonic Anhydrase I HRP Antibody at 1:1000 for 1 hr at RT. Secondary antibody: Streptavidin-HRP antibody (p/n orb348767) at 1:40000 for 30 mins at RT. Block: BlockOut (p/n orb348644). Predicted/Observed size: ~29kDa.

Quick Database Links

Gene Symbol

CA1

UniProt

P00915

RefSeq (Protein):NP_001122301.1