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Catalog Number | orb864198 |
---|---|
Category | Proteins |
Description | Canine IL-2 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Gln 237 (Accession # O62802-1). |
Target | IL-2 R alpha |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Canine |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | Asp 22 - Gln 237 |
UniProt ID | O62802 |
MW | 26.4 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Canine |
Biological Activity | Immobilized Canine IL-2 R alpha, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc, (Cat. No. IL2-H82F3) with a linear range of 0.1-10 ng/mL (QC tested). |
Expression Region | Asp 22 - Gln 237 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
Immobilized Canine IL-2 R alpha, His Tag (Cat. No. orb864198) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc, Avitag with a linear range of 0.1-10 ng/mL (QC tested).