Calpeptin

SKU: orb1300772

Description

Calpeptin is a cell-permeable, potent inhibitor of calpain proteases. It is widely used in both in vitro and in vivo research to investigate calpain's role in processes such as apoptosis, neurodegeneration, and ischemia-reperfusion injury.

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 117591-20-5
• MW: 362.46
• Purity: 97.52%
• Formula: C₂₀H₃₀N₂O₄
• SMILES: CCCC[C@H](NC(=O)[C@H](CC(C)C)NC(=O)OCc1ccccc1)C=O
• Target: Cysteine Protease,Apoptosis,Proteasome
• Solubility: DMSO:240 mg/mL (662.14 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (9.1 mM);Ethanol:67 mg/mL (184.85 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  Papainb:138 nM(ID50)|Calpain II (porcine kidney):34 nM(ID50)|Calpain I (porcine erythrocytes):52 nM(ID50)|Calpain I (human platelets):40 nM(ID50)
• In Vivo:
  In a feline right ventricular (RV) PO (RVPO) model, calpeptin (0.6 mg/kg, i.v.) blocks the activation of calpain and caspase-3, cleavage of their substrates, and cardiomyocyte programmed cell death. In a rat focal cerebral ischemia–reperfusion injury model, Calpeptin reduces the neuronal apoptosis in hippocampal CA1 sector via inhibition of the expression of Caspase-3.
• In Vitro:
  Calpeptin inhibits 20K phosphorylation in a dose-related manner in platelets stimulated by thrombin, ionomycin or collagen. In differentiating PC12 cells, Calpeptin promotes neurite elongation via inhibition of Calpain activity. In rat retinal ganglion cells, Calpeptin attenuates apoptosis, maintains normal whole-cell membrane potential, and thus provides functional neuroprotection.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, Papainb, inhibit, Apoptosis, Cathepsin, Calpain 1(porcine erythrocytes), Calpain 2(porcine kidney), Calpain 1(human platelets), Calpeptin, CysteineProtease, Cysteine Protease, Proteasome

Compound Identifiers

PubChem CID

73364