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Calcein

SKU: orb1219968

Description

Calcein is a fluorescent dye and self-quenching probe that used as an indicator of lipid vesicle leakage,and a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Images & Validation

Key Properties

CAS Number1461-15-0
MW622.53
Purity>98% (HPLC)
FormulaC30H26N2O13
SMILESOC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C22OC(=O)c3ccccc23)cc1O
TargetOthers
SolubilityDMSO : 95 mg/mL (152.6 mM); H2O : 5 mg/mL (8.03 mM; ultrasonic and adjust pH to 12 with NaOH)

Bioactivity

In Vitro
Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.

Storage & Handling

StorageStorage temperature: -20°C. Stability: ≥ 2 years
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Fluorexon

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Protocol Information