C25-140

SKU: orb1299411

Description

C25-140 is a cell-permeable small molecule that selectively disrupts the TRAF6-Ubc13 protein-protein interaction, inhibiting K63-linked polyubiquitination. It is a valuable research tool for studying NF-κB signaling in inflammation and oncology, with utility in both cellular and animal models.

Research Area

Signal Transduction

Key Properties

• CAS Number: 1358099-18-9
• MW: 457.57
• Purity: >99.99% (May vary between batches)
• Formula: C₂₆H₃₁N₇O
• SMILES: Cc1nn(c(C)c1CCC(=O)N1CCC(Cc2ccccc2)CC1)-c1ccc2nnc(C)n2n1
• Target: E1/E2/E3 Enzyme,NF-κB,TNF
• Solubility: DMSO:62.5 mg/mL (136.59 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.37 mM)

Bioactivity

• In Vitro:
  C25-140 inhibits the interaction between TRAF6 and Ubc13 in a dose-dependent manner. At concentrations of 10-30 μM over 2 hours, it effectively reduces TRAF6-mediated ubiquitin chain formation.
• Cell Research:
  Cell Line:TRAF6WT . Concentration:10 μM, 20 μM, 30 μM . Incubation Time:2 hours

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

E3 ligating enzyme, E2 conjugating enzyme, E1Enzyme, E1/E2/E3 Enzyme, E2 Enzyme, E2Enzyme, E3 Enzyme, E3Enzyme, E1 Enzyme, E1 activating enzyme, C25 140, C25140, C-25-140, C25-140, Inhibitor, NF-κB, NFkB, NFκB, NF-kB, inhibit, TNF Receptor, TNFR, Tumor Necrosis Factor Receptor, TRAF6-Ubc13, Ubiquitin conjugating enzyme, Ubiquitin activating enzyme, Ubiquitin ligase

Compound Identifiers

PubChem CID

53093165