Butylparaben

SKU: orb1310161

Description

Butylparaben

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 94-26-8
• MW: 194.23
• Purity: 98.15%
• Formula: C₁₁H₁₄O₃
• SMILES: CCCCOC(=O)C1=CC=C(O)C=C1
• Target: DNA/RNA Synthesis,Endogenous Metabolite,Antibiotic,Antibacterial
• Solubility: DMSO:60 mg/mL (308.91 mM);H2O:35 mg/mL (180.2 mM);Ethanol:36 mg/mL (185.35 mM)

Bioactivity

• In Vivo:
  Butylparaben induces collapse of Sertoli cell vimentin filaments in vivo. Exposure of a postweaning mammal to butylparaben had an adverse effect on the secretion of testosterone and in the functions of the male reproductive system.
• In Vitro:
  After butylparaben treatment, vimentin filaments in Sertolicells are disrupted. Sertoli cell cytoskeleton responds to butylparaben by exhibiting a marked collapse in vimentin filaments distribution.
• Cell Research:
  Mixed cultures of Sertoli and spermatogenic cells are prepared from three-week-old SD rats. Single cells are obtained by gravity sedimentation and use of 50% trypsin-EDTA. Cells are finally suspended at a concentration of 1 × 108cell/ml and maintained for 24 h in Dulbecco’s modified Eagle medium supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, and 10% fetal bovineserum. Thereafter, cells are maintained in serum-freemedium, which is changed every 24 h. At 72 h, spermatogenic cells are removed by treatment with hypotonic Tris buffer (20 mMTris–HCl, pH 7.4) for 5 min. The Sertoli cell-enriched cultures obtained are then maintained in serum-free medium for 24 h before being reseeded onto chamber slides. The samples are cultured for a further 5 days. Subsequently, they are exposed to 0, 1, 100 and 1000 nmol/ml butylparaben for 6 and 24 h. Some slides are stained with 0.5% toluidine blue for light microscopic observations, whereas the rest are used for vimentin immuno-histochemical analysis. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endogenous Metabolite, Butyl 4-hydroxybenzoate, Butyl paraben, Butyl parahydroxybenzoate, Butylparaben, Antibiotic, Bacterial, inhibit, Inhibitor, EndogenousMetabolite

Compound Identifiers

PubChem CID

7184