Butylated hydroxyanisole

SKU: orb1225520

Description

Butylated hydroxyanisole (BHA) is an antioxidant consisting of a mixture of two isomeric organic compounds, 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole.

Key Properties

• CAS Number: 25013-16-5
• MW: 180.25
• Purity: >98% (HPLC)
• Formula: C₁₁H₁₆O₂
• SMILES: COC1=CC(=C(O)C=C1)C(C)(C)C
• Target: Others
• Solubility: DMSO: 10 mM

Bioactivity

• In Vivo:
  Animal model: Five-week-old C57BL/6 mice (WT and Nrf2-/-). Dosage: 200 mg/kg. Administration: Oral gavage, daily, for three days. Result: Increased Nqo1 staining in hepatocytes, predominately in the pericentral region.
• In Vitro:
  Cell Proliferation Assay Cell line: NHA-SV40LT cells. Concentration: 0 μM, 25 μM, 50 μM, 75 μM, 100 μM. Incubation time: 48 hours. Result: Exerted antiproliferative effects. Cell Cycle Analysis Cell line: NHA-SV40LT cells. Concentration: 100 μM. Incubation time: 48 hours. Result: Downregulated the typical cell proliferative signaling pathways, phosphoinositide 3-kinase/protein kinase B and extracellular signal-regulated kinase 1/2.Apoptosis Analysis Cell line: NHA-SV40LT cells. Concentration: 100 μM. Incubation time: 48 hours. Result: Increased the levels of pro-apoptotic proteins, such as BAX, cytochrome c, cleaved caspase 3, and cleaved caspase 9, and decreased the level of anti-apoptotic protein BCL-XL. Western blot analysis. Cell line: NHA-SV40LT cells. Concentration: 100 μM. Incubation time: 48 hours. Result: Increased the expression of pro-apoptotic proteins and decreased the levels of anti-apoptotic proteins. Asterisks show significant effects.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

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