Buformin hydrochloride

SKU: orb1219052

Description

Buformin hydrochloride is a potent AMPK activator,an oral antidiabetic drug of the biguanide class. Buformin delays the absorption of glucose from the gastrointestinal tract increases insulin sensitivity and glucose uptake into cells and inhibits synthesis of glucose by the liver. Biguanides may antagonize the action of glucagon, thus reducing fasting glucose levels.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 1190-53-0
• MW: 193.68
• Purity: >98% (HPLC)
• Formula: C₆H₁₆ClN₅
• SMILES: CCCCN=C(N)N=C(N)N.Cl
• Target: AMPK
• Solubility: DMSO:Soluble

Bioactivity

• In Vivo:
  Animal model: Female MMTV-erbB-2 transgenic mice. Dosage: 7.6 mmol/kg. Administration: Oral administation; 7 days. Result: Inhibited mammary syngeneic tumor growth in MMTV-erbB-2 transgenic mice.
• In Vitro:
  Cell Viability Assay Cell line: ErbB-2-overexpressing SKBR3 and BT474 cells. Concentration: 0 μM, 1 μM, 3 μM, 10 μM, 30 μM, 100 μM, 300 μM, 1, 3, or 10 mM. Incubation time: 5 days. Result: Reduced cell viability in erbB-2-overexpressing breast cells. Cell Cycle Analysis Cell line: ErbB-2-overexpressing SKBR3 and BT474 cells. Concentration: 0.5 mM; 1 mM; 3 mM Incubation time: 48 hours. Result: Increased cells arresting in G0/G1 phase. Western blot analysis. Cell line: ErbB-2-overexpressing SKBR3 and BT474 cells. Concentration: 0 mM, 0.1 mM, 0.3 mM, 1 mM, or 3 mM Incubation time: 24 hours. Result: Decreased p-AMPK, p-p706S, p-ERK1/2 expression in a concentration-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 528218 | NSC-528218 | NSC528218

Quick Database Links

Gene Symbol

AMPK