Dibutyryl cAMP

SKU: orb1308676

Description

Bucladesine sodium (DC2797) is a cell-permeable cAMP analog that activates PKA and inhibits PDE, conferring anti-inflammatory properties. It is widely used in biochemical research to study cAMP signaling pathways in both cellular and animal models of inflammation.

Research Area

Cell Biology, Neuroscience, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 16980-89-5
• MW: 491.4
• Purity: >98%
• Formula: C₁₈H₂₃N₅O₈P · Na
• SMILES: [NaH].CCCC(=O)Nc1ncnc2n(cnc12)[C@@H]1O[C@@H]2COP(O)(=O)O[C@H]2[C@H]1OC(=O)CCC
• Target: Kinase
• Solubility: Soluble in DMSO (50 mg/ml); water (50 mg/ml)

Bioactivity

• In Vivo:
  METHODS: To study the anti-inflammatory activity in vivo, Bucladesine sodium (0.24-0.7 µg/kg) was administered intraperitoneally to a mouse model of copper pine demyelination once daily for seven days. RESULTS: Bucladesine had a protective effect on myelin formation. Enhanced intracellular cAMP prevented demyelination and exerted anti-inflammatory and anti-apoptotic properties in a mouse model of copper pine demyelination. METHODS: To investigate the effects on liver injury, Bucladesine sodium (0.5-500 mg/kg) was intraperitoneally injected into C57BL/6J jcl mice, and liver injury was induced by the intravenous injection of rTNF-α (1.0 µg/kg) and intraperitoneal injection of D-gal (500 mg/kg) after 1 h. The RESULTS showed that Bucladesine protected against hepatic injury in C57BL/6J jcl mice. RESULTS: Bucladesine protected mice from TNF-α/D-gal-induced liver injury.Bucladesine significantly enhanced the expression of Hsp70 in hepatocytes of D-gal/TNFα-injected mice, which was closely related to the inhibition of liver injury.
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• In Vitro:
  METHODS: Human eosinophil EoL-1 cells were treated with Bucladesine sodium (10-100 µM) for 8 days and activity was measured using PKA assay. RESULTS: Proliferation of EoL-1 cells treated with Bucladesine sodium increased in a time-dependent manner. METHODS: PC12 cells were treated with Bucladesine sodium (1 mM) for 72 h. TNF-α levels were measured by ELISA assay. RESULTS: Bucladesine sodium increased the activity of PKA.
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• Animal Research:
  Animal: Mice. Formulation: water/DMSO (9:1). Dosages: 600 nM. Administration: i.p.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bucladesine Na

Compound Identifiers

PubChem CID

154809571