BTB09089

SKU: orb1741369

Description

BTB09089 is a selective agonist for the TDAG8/GPR65 receptor. It upregulates TDAG8 expression and modulates cytokine secretion in immune cells like T cells and macrophages, serving as a valuable research tool for studying GPR65's role in inflammatory and autoimmune disease models both in vitro and in vivo.

Research Area

Metabolism Research, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 245728-44-3
• MW: 387.31
• Purity: 99.78%
• Formula: C₁₄H₁₂Cl₂N₄OS₂
• SMILES: CN1C2=C(SC(SCC3=C(Cl)C=C(Cl)C=C3)=N1)C(=O)N(C)N=C2
• Target: DNA,GPCR,Others
• Solubility: DMSO:14.7 mg/mL (37.95 mM)

Bioactivity

• In Vivo:
  BTB09089 (5-20 μM, 8 μL; i.c.v.; administered 6 hours prior to MCAO) reduces cerebral ischemia-reperfusion injury in rats.
• In Vitro:
  BTB09089 (0-18 μM; 30 min) significantly increases cAMP accumulation in HEK293 cells expressing hTDAG8 and mTDAG8, but not in control HEK293 cells. It does not enhance cAMP accumulation in HEK293 cells expressing hGPR4 or inositol phosphate accumulation in HEK293 cells expressing hOGR1. BTB09089 (0-18 μM; 30 min) enhances cAMP accumulation dose-dependently at pH 7.0-7.9, but not at pH 6.5. BTB09089 (1-5 μM; 20 h) inhibits IL-2 production in WT mouse splenocytes dose-dependently, without affecting IL-2 production in TDAG8 KO mice or cell viability. BTB09089 (1-5 μM; 18 h) inhibits TNF-α and IL-6 production stimulated by LPS while enhancing IL-10 production in TG-PEC induced by LPS stimulation with sulfated ester.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPR65, GPR65 agonist, GPR65 agonist (BTB09089), DNA, BTB 09089, BTB09089, BTB-09089

Compound Identifiers

PubChem CID

2801217