BSH-IN-1

SKU: orb1710199

Description

BSH-IN-1 is a potent, covalent inhibitor of gut bacterial bile salt hydrolases (BSHs), demonstrating high potency with IC50 values of 108 nM and 427 nM against B. longum and B. theta BSH, respectively. This tool compound enables research into microbiome function, supporting in vitro and in vivo studies on host metabolism and bile acid signaling.

Research Area

Infectious Disease & Virology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 2553217-91-5
• MW: 408.59
• Purity: 99.89% (May vary between batches)
• Formula: C₂₅H₄₁FO₃
• SMILES: [H][C@]1([C@@H](CCC(CF)=O)C)CC[C@]2([C@@]3([C@@H](C[C@@]4(C[C@@H](CC[C@@]4([C@]3(CC[C@]12C)[H])C)O)[H])O)[H])[H]
• Target: Antibacterial,Potassium Channel
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.89 mM);DMSO:40 mg/mL (97.9 mM)

Bioactivity

• In Vivo:
  In C57BL/6 mice, BSH-IN-1 (10 mg/kg; a single dose; gavage）inhibits BSH activity and can be gut-restricted.
• In Vitro:
  BSH-IN-1 also inhibits BSH activity in growing cultures of Gram-negative (Bacteroides fragilis ATCC 25285, B. theta VPI-5482, and Bacteroides vulgatus ATCC 8482) and Gram-positive (C. perfringens ATCC 13124, Lactobacillus plantarum WCFS1, and Bifidobacterium adolescentis L2–32) bacteria. BSH-IN-1 also is a potent BSH inhibitor in growing bacterial cultures with IC50s of 237 nM and 1070 nM for B. adolescentis and B. theta, respectively.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BSHIN1, BSH-IN-1, BSH IN 1, Bile salt hydrolase (BSH), PotassiumChannel, Potassium Channel

Compound Identifiers

PubChem CID

145713829