Brilliant blue G-250

SKU: orb1306551

Description

Coomassie Brilliant Blue G-250 (Acid Blue 90) is a selective P2X7 receptor antagonist. It is widely used in biochemical research to study purinergic signaling, inflammation, and cell death pathways in both in vitro cellular assays and in vivo experimental models.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 6104-58-1
• MW: 854.02
• Purity: ≥98% (May vary between batches)
• Formula: C₄₇H₄₈N₃NaO₇S₂
• SMILES: O=S(C1=CC=CC(CN(CC)C2=CC=C(/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C(C)=C/C(C=C/5)=[N+](CC)\CC6=CC=CC(S(=O)([O-])=O)=C6)C(C)=C2)=C1)([O-])=O.[Na+]
• Target: P2X Receptor
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:0.5 mg/mL (0.59 mM);DMSO:8.55 mg/mL (10.01 mM)

Bioactivity

• In Vitro:
  Brilliant Blue G-250 is used for protein staining after polyacrylamide gel electrophoresis Instructions 1. The protein electrophoresis gel is fixed in 50% methanol, 10% acetic acid, and 40% aqueous solution, and shaken at a low speed on a horizontal shaker for 30 min to overnight. 2. Remove the fixative and replace with 0.25% Coomassie Brilliant Blue R250 staining solution (0.25% R250 dissolved in 50% methanol, 10% acetic acid, and 40% aqueous solution) to completely cover the gel and stain for 2-4 h. Until the gel is stained uniformly blue. Note: When the gel is invisible to the naked eye in the staining solution, it means that the staining is complete, otherwise the gel area looks lighter in color compared to the dark staining solution. 3. Place the gel back in 5% methanol, 7.5% acetic acid, and 87.5% aqueous solution for 4-24 h for decolorization. The protein bands will be visible after about 1-2 hours, and the destaining process will not be completed until the background becomes transparent. The destaining solution can be changed 2-4 times in the middle. 4. Store the gel in 7% acetic acid. It can also be stored in water or dry.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

P2XReceptor, P2X7, SDS-PAGE, P2X Receptor, P2×7R, Inhibitor, NLRP3 inflammasome, inhibit, dye, Brilliant Blue G, Brilliant blue G 250, Brilliant blue G250, Brilliant blue G-250, Acid blue 90, Coomassie Brilliant Blue G, Bradford protein, BBG

Compound Identifiers

PubChem CID

61363