BrdU Antibody

SKU: orb344495

Description

BrdU antibody

Images & Validation

• Tested Applications: DOT, ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:2000 - 1:10000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500-1:3000, WB: 1:2000 - 1:5000
• Reactivity: Other
• Application Notes:
  Anti-BrdU Antibody has been tested immunofluorescence and slot blot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 29G6.E8
• Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
• Purity: Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody reacts strongly with BrdU. Cross-reactivity is observed with CldU and IdU.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU