BQU57

SKU: orb1305856

Description

BQU57 is a selective small molecule inhibitor of the Ral GTPases, demonstrating specificity over related proteins Ras and Rho. It has been shown to inhibit tumor growth in xenograft models, making it a valuable research tool for studying Ral signaling in cancer biology both in vitro and in vivo.

Research Area

Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 1637739-82-2
• MW: 334.3
• Purity: 98.36%
• Formula: C₁₆H₁₃F₃N₄O
• SMILES: Cc1nn(C)c2OC(N)=C(C#N)C(c12)c1ccc(cc1)C(F)(F)F
• Target: Ras,GTPase
• Solubility: Ethanol:16 mg/mL (47.86 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.98 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:40 mg/mL (119.65 mM)

Bioactivity

• In Vivo:
  BQU57 inhibited RalA and RalB activation in H2122 and H358 cell lines, resulting in cell growth inhibition.
• In Vitro:
  BQU57 inhibited RalA and RalB activation in H2122 and H358 cell lines, resulting in cell growth inhibition.
• Cell Research:
  Growth inhibition on human lung cancer cells by the compounds are measured under anchorage-independent conditions in soft agar. Cells are seeded into 6-well plates (coated with a base layer made of 2.0 mL of 1% low-melting-point agarose) at 15,000 cells per well in 3.0 mL of 0.4% low-melting-point agarose containing various concentration of drug. Two to four weeks (depending on cell line) after incubation, cells are stained with 1.0 mg/mL Nitro Blue Tetrazolium and colonies are counted under a microscope. The IC50 values are defined as the concentration of drug that resulted in 50% reduction in colony number compared to DMSO treated control. After 48 hr, cells are subjected to the soft agar colony formation assay. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BQU 57, BQU57, BQU-57, inhibit, Inhibitor, GTPase Ral, Ras

Compound Identifiers

PubChem CID

77845606