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Catalog Number | orb1496492 |
---|---|
Category | Assays and Kits |
Description | Bovine VA ELISA Kit |
Reactivity | Bovine |
Range | 1.57-100 ng/mL |
Assay Time | 2.5h |
Concentration | 100 ng/mL |
Sensitivity | 0.55 ng/mL |
Alternative names | VA Read more... |
Note | For research use only |
Application notes | standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle VA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle VA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle VA in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Bovine | |
6.25 ng/mL-100 ng/mL | |
4.672 ng/mL |