ALB Antibody

SKU: orb2652803

Description

ALB Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000-1:10,000
• Reactivity: Bovine
• Application Notes:
  Anti-Bovine Serum Albumin (BSA) antibody has been tested by western bot and ELISA and is suitable in dot blot, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with bovine serum albumin.
• Target: ALB
• Purity: Bovine Serum Albumin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum). Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody, rabbit Anti-BSA, rabbit anti-bovine serum albumin

ELISA results of purified Rabbit anti-Bovine Serum Albumin Antibody tested against Bovine Serum Albumin. Each well was coated in duplicate with 1.0 µg of the antigen. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Western Blot of Rabbit Anti-BSA Antibody. Lane 1: Molecular Weight Marker. Lane 2: BSA. Primary Antibody: Rabbit anti-Bovine Serum Albumin at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit Peroxidase conjugated (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: 5% goat serum.

Quick Database Links

Gene Symbol

ALB

UniProt

P02769

RefSeq:AAA51411.1