Bovine Collagen Type I protein

• Catalog Number: orb750375
• Category: Proteins
• Description: Bovine Collagen Type I protein
• Target: COL1A1
• Conjugation: Unconjugated
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: Bovine Collagen Type 1 has been prepared from Bovine Placenta and is chromatographically and immunologically pure. Bovine Collagen Type 1 reacts with anti-Collagen Type I. Reaction with Rockland’s anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).
• UniProt ID: P02453
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Dilution range: IF: 1:100-1:500, WB: 1:1000
• Application notes: Bovine Collagen Type I purified protein standard is used as a control for SDS-PAGE, Western Blot, ELISA, immunoprecipitation, and for other immunological assays. Specific conditions should be optimized by user. Collagen type I is recognized by type specific Anti-Collagen antibodies that recognize a native three-dimensional structure.
• Source: Bovine
• Biological Origin: Bovine
• Storage: Store vial at 4° C prior to opening. Do not freeze. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Type I collagen protein, collagen 1 protein, bovin
• Note: For research use only
• NCBI: NP_001029211.1

Aggf1 interacts with and modulates SMAD7 activity. (D) Primary HSCs were infected with Ad-V or Ad-AGGF1 and then transfected with either siRNA targeting SMAD7 or scrambled siRNA (SCR). Expression of fibrogenic genes was examined by Western and qPCR (not shown).

Aggf1 suppresses liver fibrosis following injury in mice. (B) C57/BL6 mice were injected via tail vein adenovirus carrying either Aggf1 expression vector (Ad-AGGF1) or an empty vector (Ad-V) followed by CCl4 injection to induce liver injury. Expression of fibrogenic genes was examined by Western and qPCR (not shown).

C57/BL mice were injected peritoneally with chaetocin (25 mg/kg) or DMSO 2 days prior to the LAD procedure. The mice were sacrificed 4 weeks after the surgery. (B) Expression of pro-fibrogenic genes was examined by Western blotting and qPCR (not Shown).

SDS-PAGE Results of Bovine Collagen Type I. Lane 1: Bovine Collagen Type I -Reduced [10 µg]. Lane 2: Opal Prestained Molecular Weight Marker Lane 3: Bovine Collagen Type I -Non-Reduced [10 µg]. 4-20% Gel, Coomassie Stained.