Bohemine

SKU: orb1225820

Description

Bohemine is a cyclin-dependent kinase inhibitor.

Key Properties

• CAS Number: 189232-42-6
• MW: 340.42
• Purity: >98% (HPLC)
• Formula: C₁₈H₂₄N₆O
• SMILES: CC(N1C=NC2=C1N=C(NCCCO)N=C2NCC3=CC=CC=C3)C
• Target: CDK
• Solubility: DMSO: 10 mM

Bioactivity

• In Vivo:
  Bohemine (50 mg/kg; intravenous injection; BALB/c mice) treatment shows Cmax is 72, 308 nM, observed clearance is 0.23 L/h and T1/2 is 1.39 h. Animal model: BALB/c mice bearing the colon 26 murine tumor. Dosage: 50 mg/kg. Administration: Intravenous injection (Pharmacokinetic Analysis). Result: Cmax is 72, 308 nM, observed clearance is 0.23 L/h and T1/2 is 1.39 h.
• In Vitro:
  Bohemine (0-30 μM; 72 hours; ME-750 cells) treatment inhibits cell growth. Addition of Bohemine at concentrations in the range of 1-10 μM results in a short-term arrest of growth and of monoclonal antibody production. The short-term suppression of cell functions is followed by a significant temporary increase of specific growth rate and of specific production rate. Hybridoma cells are retarded both at the G1/S boundary and at the G2/M boundary, depending on Bohemine (0-30 μM) concentration. T-lymphoblastic cell line CEM is treated by Bohemine, five proteins are found to be downregulated, namely α-enolase, triosephosphate isomerase, initiation factor 5A, and α- and β-subunits of Rho GDP-dissociation inhibitor 1. These proteins play significant roles in glycolysis, proteosynthesis, and in cytoskeleton rearrangement. Bohemine inhibits growth of human tumor cell lines with an IC50 of 27 μM. Cell Viability Assay Cell line: Mouse hybridoma ME-750 cells. Concentration: 0 μM, 1 μM, 3 μM, 10 μM and 30 μM. Incubation time: 72 hours. Result: At 10 μM and 30 μM concentrations, the viable cell count was significantly lower with respect to control, i. e., 77% and 48%, respectively.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bohemine

Quick Database Links

Gene Symbol

CDK