BMS-5

SKU: orb1302722

Description

BMS-5 (LIMKi 3) is a highly potent and selective small molecule inhibitor of LIM kinase, demonstrating IC50 values of 7 nM against LIMK1 and 8 nM against LIMK2. This compound is a valuable research tool for studying cytoskeletal dynamics, cell motility, and metastasis in both cellular and animal models of cancer and neurological disorders.

Research Area

Cell Biology

Key Properties

• CAS Number: 1338247-35-0
• MW: 431.29
• Purity: 99.88% (May vary between batches)
• Formula: C₁₇H₁₄Cl₂F₂N₄OS
• SMILES: CC(C)C(=O)Nc1ncc(s1)-c1cc(nn1-c1c(Cl)cccc1Cl)C(F)F
• Target: LIM Kinase
• Solubility: DMSO:55 mg/mL (127.52 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.64 mM)

Bioactivity

• Target IC50:
  LIMK2:8 nM|LIMK1:7 nM
• In Vivo:
  BMS-5 (20 or 200 μM/side) was administered bilaterally into the hippocampi of rats immediately following contextual fear conditioning training. Memory consolidation was assessed 48 hours after the conditioning through tests measuring freezing behavior. Analysis revealed that rats receiving the 200 μM dose of BMS-5 exhibited significantly reduced freezing compared to those treated with 20 μM and the control group [P].
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• In Vitro:
  BMS-5 inhibits cofilin-Ser3 phosphorylation in a dose-dependent manner in Nf2ΔEx2 mouse Schwann cells (MSCs) with an IC50 of ~2 μM. BMS-5 reduces Nf2ΔEx2 MSC viability in a dose-dependent manner with an IC50 of 3.9 μM, but does not significantly reduce the viability of control Nf2flox2/flox2 MSCs at equivalent BMS-5 concentrations. At 10 μM BMS-5, Nf2ΔEx2 MSC viability is 40% compared to 83% for controls .
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• Cell Research:
  Cell membrane asymmetry is measured. Nf2ΔEx2 MSCs plated in a 6-well format are incubated with 2 μM BMS-5 or DMSO vehicle for 24 hrs. Cell are harvested and assayed. Plasma membrane asymmetry is evaluated with the Violet ratiometric assay by flow cytometry .
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• Animal Research:
  Rats

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, LIM Kinase, LIM Kinase (LIMK), LIMK1, LIMK2, LIMKi 3, LIMKi3, LIMKi-3, LIMKinase, LIMKs, BMS 5, BMS5, BMS-5

Compound Identifiers

PubChem CID

56965901