BMH-21

SKU: orb1307493

Description

BMH-21

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 896705-16-1
• MW: 360.41
• Purity: 99.84%
• Formula: C₂₁H₂₀N₄O₂
• SMILES: CN(C)CCNC(=O)c1cccn2c1nc1cc3ccccc3cc1c2=O
• Target: DNA/RNA Synthesis
• Solubility: Ethanol:2 mg/mL (5.55 mM);DMSO:< 1 mg/mL (insoluble or slightly soluble);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  RNA polymerase I:10-90 nM
• In Vivo:
  I the U2OS cancer cell line, BMH-21 induce the translocation of NCL IC50 0.07 μM) an the degradation of RPA194 IC50 0.05 μM). BMH-21 triggers proteasomal destruction dependent on RPA194, where RPA194 in the large catalytic subunit protein o the Pol I complex. By inducing nucleolar stress, BMH-21 also effectively inhibits cellular activity.
• In Vitro:
  In xenograft models using mice, BMH-21 can inhibit tumor growth.
• Cell Research:
  The cells are maintained at 37 °C in a humidified atmosphere containing 5% CO2. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h wit the compounds. Viability is determined using WST-1 cell proliferation reagent. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

damage, DNASynthesis, DNA/RNA Synthesis, DNA Synthesis, DNA, BMH21, BMH-21, BMH 21, inhibit, nucleolus, intercalation, Inhibitor, transcription, polymerase, RNASynthesis, RNA, RNA polymerase I, RNA Synthesis, response

Compound Identifiers

PubChem CID

3508054