BMH-21

SKU: orb1307493

Description

BMH-21 is a small molecule that selectively intercalates into ribosomal DNA, thereby inhibiting RNA polymerase I transcription. It is a valuable research tool for studying nucleolar stress and ribosome biogenesis in both cellular and animal models, without inducing DNA damage signaling via H2AX phosphorylation.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 896705-16-1
• MW: 360.41
• Purity: 99.84% (May vary between batches)
• Formula: C₂₁H₂₀N₄O₂
• SMILES: CN(C)CCNC(=O)c1cccn2c1nc1cc3ccccc3cc1c2=O
• Target: DNA/RNA Synthesis
• Solubility: Ethanol:2 mg/mL (5.55 mM);DMSO:< 1 mg/mL (insoluble or slightly soluble);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  RNA polymerase I:10-90 nM
• In Vivo:
  In the U2OS cancer cell line, BMH-21 induces the translocation of NCL (IC50: 0.07 μM) and the degradation of RPA194 (IC50: 0.05 μM). BMH-21 triggers proteasomal destruction dependent on RPA194, where RPA194 is the large catalytic subunit protein of the Pol I complex. By inducing nucleolar stress, BMH-21 also effectively inhibits cellular activity.
• In Vitro:
  In xenograft models using mice, BMH-21 can inhibit tumor growth.
• Cell Research:
  The cells are maintained at 37 °C in a humidified atmosphere containing 5% CO2. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h with the compounds. Viability is determined using WST-1 cell proliferation reagent. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

damage, DNASynthesis, DNA/RNA Synthesis, DNA Synthesis, DNA, BMH21, BMH-21, BMH 21, inhibit, nucleolus, intercalation, Inhibitor, transcription, polymerase, RNASynthesis, RNA, RNA polymerase I, RNA Synthesis, response

Compound Identifiers

PubChem CID

3508054