Biotinylated Syndecan-1 Recombinant Protein

• Catalog Number: orb1231483
• Category: Proteins
• Description: Biotinylated Syndecan-1 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His Tag & Avi Tag
• Form/Appearance: Lyophilized
• Purity: > 90% as determined by SDS-PAGE.
• MW: 27.5 kDa
• Protein Sequence: Gln 23 - Gly 254
• Source: HEK293 cells
• Storage: Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: SDC1,Syndecan-1,CD138,SYND1,SDC
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 27.5 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
• Expiration Date: 6 months from date of receipt.

Biotinylated Human Syndecan-1, His, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Biotinylated Human Syndecan-1, His, Avitag at 1 ug/ml (100 uL/well) on streptavidin (0.5 ug/well) plate, can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL.

Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 ug/ml (100 uL/well) onstreptavidin (0.1 ug/well) plate, can bind Biotinylated Human Syndecan-1, His, Avitag with a linear range of 2-63 ng/mL.