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Biotinylated Human TL1A

SKU: orb2992948

Description

Biotinylated Recombinant Human Tumor necrosis factor ligand superfamily member 15 is produced by our Mammalian expression system and the target gene encoding Leu72-Leu251 is expressed with a 6His, Avi tag at N-terminus.

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Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityELISA: Immobilized Biotinylated Human TL1A (N-6His-Avi) (Cat#orb2992948) at 2μg/ml (100 μl/well) can bind Human TL1A Antibody (9H5) (Cat#orb2992933). The ED50 of Human TL1A Antibody (9H5) (Cat#orb2992933) is 3.44 ng/ml. (Regularly tested). BLI: Loaded Biotinylated Human TL1A (N-6His-Avi) (Cat#orb2992948) on HIS1K Biosensor, can bind Human TL1A Antibody (9H5) (Cat#orb2992933) with an affinity constant of 3.01 pM as determined in BLI assay. (Regularly tested)
TagN-6His-Avi
Molecular WeightPredicted: 23.1 KDa. Observed: 25-35 KDa, reducing conditions
Expression RegionLeu72-Leu251
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationUnconjugated

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Tumor Necrosis Factor Ligand Superfamily Member 15; TNF Ligand-Related Molecule 1; Vascular Endothelial Cell Growth Inhibitor; TNFSF15; TL1; VEGI

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Biotinylated Human TL1A (orb2992948)

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