Biotinylated Human TGF-beta 3

SKU: orb2993088

Description

Biotinylated Recombinant Human Transforming Growth Factor Beta-3 Proprotein is produced by our Mammalian expression system and the target gene encoding Ala301-­Ser412 (Tyr340Phe) is expressed with a Avi tag at the N-terminus.

Key Properties

• Expression System: Human cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human TGFBR2-Fc(Cat#orb3002421) at 2μg/ml (100 μl/well)can bind Biotinylated Human TGF-beta 3-Avi (Cat#orb2993088). The ED50 of Biotinylated Human TGF-beta 3-Avi (Cat#orb2993088)is 2.05 ng/ml.. Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.37ng/ml.
• Tag: N-Avi
• Molecular Weight: Predicted: 14.5 KDa. Observed: 10-14 KDa, reducing conditions
• Expression Region: Ala301-­Ser412 (Tyr340Phe)
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 4mM HCl.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

TGFB3; ARVD; TGF-beta3

Quick Database Links

UniProt

P10600