Biotinylated Human TfR

SKU: orb2992761

Description

Recombinant Human Transferrin receptor protein 1 is produced by our Mammalian expression system and the target gene encoding Cys89-Phe760 is expressed with a 6His, Avi tag at N-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human Transferrin (C-6His) (Cat#orb2994488) at 5μg/ml (100 μl/well) can bind Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761). The ED50 of Biotinylated Human TfR (N-6His-Avi)(Cat#orb2992761) is 22.33 ng/ml. (Regularly tested). BLI: Loaded Anti-Human TFRC mAb (Human IgG1) on Pro-A Biosensor, can bind Biotinylated Human TfR (N-6His-Avi) (Cat#orb2992761) with an affinity constant of 1.69 nM as determined in BLI assay. (Regularly tested)
• Tag: N-6His-Avi
• Molecular Weight: Predicted: 78.1 KDa. Observed: 80-95 KDa, reducing conditions
• Expression Region: Cys89-Phe760
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 0.01% Tween80, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Transferrin receptor protein 1; TR; TfR; Trfr; T9; p90

Quick Database Links

UniProt

P02786