Biotinylated Human SIRPA

SKU: orb2993923

Description

Biotinylated Recombinant Human Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His, Avi tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human SIRPA mAb-Fc(Cat#orb2993142) on Protein A Biosensor, can bind Biotinylated Human SIRPA-His(Cat#orb2993923) with an affinity constant of 5.57 nM as determined in BLI assay.
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 39.9 KDa. Observed: 50-80 KDa, reducing conditions
• Expression Region: Glu31-Arg370
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; SHP Substrate 1; SHPS-1; Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs; Bit; CD172 Antigen-Like Family Member A; Inhibitory Feceptor SHPS-1; Macrophage Fusion Receptor; MyD-1 Antigen; Signal-Regulatory Protein Alpha-1; Sirp-Alpha-1; Signal-Regulatory Protein Alpha-2; Sirp-Alpha-2; Signal-Regulatory Protein Alpha-3; Sirp-Alpha-3; p84; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP

Quick Database Links

UniProt

P78324