Biotinylated Human PD-1

SKU: orb2993936

Description

Biotinylated Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a 6His, Avi tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human PD-1-His-Avi(Cat#orb2993936) at 2μg/ml (100 μl/well) can bind Anti-Human PD-1 mAb(Cat#GMP-A085). The ED50 of Human Anti-Human PD-1 mAb(Cat#GMP-A085) is 8.83 ng/ml. (Regularly tested). BLI: Loaded Anti-Human PD-1 mAb-Fc(Cat#GMP-A085) on Protein A Biosensor, can bind Biotinylated Human PD-1-His(Cat#orb2993936) with an affinity constant of 1.54 nM as determined in BLI assay.
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 19.2 KDa. Observed: 35-45 KDa, reducing conditions
• Expression Region: Pro21-Gln167
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Programmed cell death protein 1; hPD-1; PDCD1; CD279

Quick Database Links

UniProt

Q15116