Biotinylated Human LDL R

SKU: orb2994702

Description

Biotinylated Recombinant Human Low-Density Lipoprotein Receptor is produced by our Mammalian expression system and the target gene encoding Ala22-Arg788 is expressed with a Avi, 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: BLI: Loaded Biotinylated Human LDL R-Avi-His(Cat#orb2994702) on SA Biosensor, can bind Mouse PCSK9-His(Cat#orb2993451) with an affinity constant of 4.97 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Avi-6His
• Molecular Weight: Predicted: 88.4 KDa. Observed: 95-140 KDa, reducing conditions
• Expression Region: Ala22-Arg788
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Low-Density Lipoprotein Receptor; LDL Receptor; LDLR

Quick Database Links

UniProt

P01130