Biotinylated Human IL-4RA

SKU: orb2992716

Description

Biotinylated Recombinant Human Interleukin-4 receptor subunit alpha is produced by our Mammalian expression system and the target gene encoding Met26-His232 is expressed with a Avi, 6His tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Biotinylated Human IL-4RA (C-Avi-6His) (Cat#orb2992716) at 2 μg/ml (100 μl/well) can bind Recombinant Human IL-4 (C-Fc) (Cat#orb3002348). The ED50 of Recombinant Human IL-4 (C-Fc) (Cat#orb3002348) is 17.42 ng/ml. (Regularly tested). Immobilized Recombinant Human IL-4 (E. coli) (Cat#orb3002443) at 5 μg/ml (100 μl/well) can bind Biotinylated Human IL-4RA (C-Avi-6His) (Cat#orb2992716). The ED50 of Biotinylated Human IL-4RA (C-Avi-6His) (Cat#orb2992716) is 94.2 ng/ml. (Regularly tested).
• Tag: C-Avi-6His
• Molecular Weight: Predicted: 26.2 KDa. Observed: 35-65 KDa, reducing conditions
• Expression Region: Met26-His232
• Purity: SDS-PAGE: Greater than 95% as determined by SDS-PAGE. (QC verified)
• Endotoxins: ＜ 1 EU/μg as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA

Quick Database Links

UniProt

P24394