Recombinant Human IgG1 Fc Protein (Biotin)

SKU: orb2993718

Description

Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu,Leu241Met) is expressed with a Avi tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Biotinylated Human IgG1 Fc(Cat#orb2993718) at 2μg/ml (100 μl/well) can bind Recombinant Protein G(Cat#orb2993145). The ED50 of Recombinant Protein G(Cat#orb2993145) is 49.41 ng/ml. (Regularly tested). BLI: Loaded Biotinylated Human IgG1 Fc-His-Avi(Cat#orb2993718) on SA Biosensor, can bind Protein G-Fc(Cat#orb2993145) with an affinity constant of <1 pM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human IgG1 Fc (Cat#orb2993718) on SA Biosensor, can bind Recombinant Human CD64(C-6His)(Cat#orb2994349) with an affinity constant of 1.23 nM as determined in BLI assay. (Regularly tested)
• Tag: C-Avi
• Molecular Weight: Predicted: 27.7 KDa. Observed: 33 KDa, reducing conditions
• Expression Region: Asp104-Lys330(Asp239Glu,Leu241Met)
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

g gamma-1 chain C region; IGHG1

Quick Database Links

UniProt

P01857