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Biotinylated Human HLA-A*0201 GP100 V2 complex Protein (C-Avi-10His)

SKU: orb2992881

Description

Biotinylated Human HLA-A*0201 GP100 V2 complex Protein (C-Avi-10His) is produced by our Mammalian expression system and the target gene encoding (YLEPGPVTV)(Ala288Val)&Ile21-Met119&Gly25-Ile308 is expressed with a Avi, 10His tag at C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
TagC-Avi-10His
Molecular WeightPredicted: 52.8 KDa. Observed: 53-63 KDa, reducing conditions
Expression Region(YLEPGPVTV)(Ala288Val)&Ile21-Met119&Gly25-Ile308
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.
ConjugationBiotin

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 1 months.
Form/AppearanceLyophilized from a 0.2 μm filtered solution of 20mM PB, 500mM NaCl, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
DisclaimerFor research use only

Alternative Names

HLA-A*0201 GP100 complex Protein
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Biotinylated Human HLA-A*0201 GP100 V2 complex Protein (C-Avi-10His) (orb2992881)

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