Recombinant Human Frizzled-8 Protein (Biotin)

SKU: orb2993701

Description

Biotinylated Recombinant Human Frizzled-8 is produced by our Mammalian expression system and the target gene encoding Ala28-Pro172 is expressed with a 6His, Avi tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Biotinylated Human Frizzled-8 (C-6His-Avi)(Cat#orb2993701) at 2 μg/ml (100 μl/well) can bind Human Frizzled receptors Antibody(Vantictumab,Research Grade)(Cat#orb2992588). The ED50 of Human Frizzled receptors Antibody(Vantictumab,Research Grade)(Cat#orb2992588) is 9.67 ng/ml.(Regularly tested).. BLI: Loaded Human Frizzled receptors Antibody (Vantictumab, Research Grade) (Cat#orb2992588) on Pro-A Biosensor, can bind Biotinylated Human Frizzled-8 (C-6His-Avi) (Cat#orb2993701) with an affinity constant of 0.88 μM as determined in BLI assay. (Regularly tested)
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 19.1 KDa. Observed: 25-35 KDa, reducing conditions
• Expression Region: Ala28-Pro172
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8

Quick Database Links

UniProt

Q9H461