Recombinant Cynomolgus CA125 / MUC16 Protein (Biotin)

SKU: orb2992947

Description

Biotinylated Recombinant Cynomolgus Mucin-16 is produced by our Mammalian expression system and the target gene encoding Gly11545-Pro12188 is expressed with a 6His, Avi tag at C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Cynomolgus
• Biological Activity: ELISA: Immobilized Biotinylated Cynomolgus MUC16 (C-6His-Avi)(Cat#orb2992947) at 5 μg/ml (100 μl/well) can bind Recombinant Human Mesothelin (C-Fc)(Cat#orb2994279). The ED50 of Recombinant Human Mesothelin (C-Fc)(Cat#orb2994279) is 0.405 ug/ml. (Regularly tested). BLI: Loaded Recombinant Human Mesothelin (C-Fc) (Cat#orb2994279) on Pro-A Biosensor, can bind Biotinylated Cynomolgus MUC16 (C-6His-Avi) (Cat#orb2992947) with an affinity constant of 15.3 pM as determined in BLI assay. (Regularly tested)
• Tag: C-6His-Avi
• Molecular Weight: Predicted: 74.6 KDa. Observed: 90-150 KDa
• Expression Region: Gly11545-Pro12188
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CA125 ovarian cancer antigen; CA125; CA-125; CA125; MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16

Quick Database Links

UniProt

XP_045234963.1