Biotin Conjugation Kit

SKU: orb3068313

Description

Biotin Conjugation Kit uses a simple and quick process for Biotin labeling/conjugation of antibodies. It can also be used to conjugate other proteins or peptides.

Images & Validation

• Application Notes:
  Equilibrate all materials and prepared reagents to room temperature prior to use. Note: If the protein contains free amino groups (Tris, amino acids or other interferents), please centrifuge it 2-3 times (8,000 × g, 10 min) using the Filtration Tube to ensure that it is removed. For 1 mg antibody 1. Add 200 μl of Reaction Buffer into each 1 ml of antibody (e.g. antibody concentration 1 mg/ml) to be labeled and mix gently. (Reduce the amount of Reaction Buffer at the same scale) 2. Add 30 μl of 10 mM NHS Biotin into the protein solution, gently blow and mix fully. Replace cap on the vial and leave standing for 30 minutes in the dark at 37°C hot air circulating oven. 3. After incubating, add 5 μl of Quencher Reagent for each 30 μl of 10 mM NHS Biotin used and mix gently (Reduce the amount of Quencher Reagent at the same scale). The conjugates can be used after 10 minutes. 4. Add an appropriate amount of 1×PBS into the above reaction solution, cover the cap after matching with the collection tube, and centrifuge for 10-30 min at the speed of 12,000 × g. Discard the liquid in the collection tube, replenish 1×PBS in the filter device, and repeat the centrifugal ultrafiltration operation for 2-3 times. 5. Invert the filter device in another collection tube and centrifuge at 1000 × g for 2 mins. Collect the solution in the collection tube.

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C and protect from light for 2 years.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only