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    Biotin-11-ddUTP

    Biotin-11-ddUTP

    Catalog Number: orb64038

    DispatchUsually dispatched within 5-10 working days
    $ 547.00
    Catalog Numberorb64038
    CategoryTools
    DescriptionBiotin-X-5-Propargylamino-ddUTP
    Concentration1.0 mM-1.1 mM
    Form/Appearancefiltered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
    Purity≥ 95% (HPLC)
    MWTheoretical MW: 844.66 g/mol (free acid); Detected MW: 844.17 g/mol (free acid)
    SMILESC1C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)C#CCNC(=O)CCCCCNC(=O)CCCC[C@H]1[C@@H]2[C@H](CS1)NC(=O)N2
    FormulaC28H43N6O16P3S
    Storagestore at -20 °C
    NoteFor research use only
    Application notesSNP-analysis; DNA sequencing; 3'end labeling.
    Spectroscopic Propertie: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
    Expiration Date12 months from date of receipt.
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    Biotin-11-ddUTP (orb64038)

    We performed CHIP-SICAP, a modified CHIP protocol that involves stringent wash to remove non-specific chromatin-associated proteins. So, labeling DNA with biotin followed by steptavidin is key to achieving high sensitivity. We evaluated the results by qPCR. We got great results with as little as four times of the cells as prescribed by other CHIP SICAP users.

    - Charlesantony Aruljothi

    Applications

    ChIP

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