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Biotin-11-ddUTP

Biotin-11-ddUTP

Catalog Number: orb64038

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5 x 25 μl$ 590.00
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Product Properties
Catalog Numberorb64038
CategoryTools
DescriptionBiotin-X-5-Propargylamino-ddUTP
Form/Appearancefiltered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Concentration1.0 mM-1.1 mM
Purity≥ 95% (HPLC)
MWTheoretical MW: 844.66 g/mol (free acid); Detected MW: 844.17 g/mol (free acid)
Application notesSNP-analysis; DNA sequencing; 3'end labeling.
Spectroscopic Propertie: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
FormulaC28H43N6O16P3S
SMILESC1C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)C#CCNC(=O)CCCCCNC(=O)CCCC[C@H]1[C@@H]2[C@H](CS1)NC(=O)N2
Storagestore at -20 °C
NoteFor research use only
Expiration Date12 months from date of receipt.
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Applications: ChIP| Reactivity: Other

We performed CHIP-SICAP, a modified CHIP protocol that involves stringent wash to remove non-specific chromatin-associated proteins. So, labeling DNA with biotin followed by steptavidin is key to achieving high sensitivity. We evaluated the results by qPCR. We got great results with as little as four times of the cells as prescribed by other CHIP SICAP users.

- Charlesantony Aruljothi