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| Catalog Number | orb64038 |
|---|---|
| Category | Tools |
| Description | Biotin-X-5-Propargylamino-ddUTP |
| Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
| Concentration | 1.0 mM-1.1 mM |
| Purity | ≥ 95% (HPLC) |
| MW | Theoretical MW: 844.66 g/mol (free acid); Detected MW: 844.17 g/mol (free acid) |
| Application notes | SNP-analysis; DNA sequencing; 3'end labeling. Spectroscopic Propertie: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Formula | C28H43N6O16P3S |
| SMILES | C1C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)C#CCNC(=O)CCCCCNC(=O)CCCC[C@H]1[C@@H]2[C@H](CS1)NC(=O)N2 |
| Storage | store at -20 °C |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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We performed CHIP-SICAP, a modified CHIP protocol that involves stringent wash to remove non-specific chromatin-associated proteins. So, labeling DNA with biotin followed by steptavidin is key to achieving high sensitivity. We evaluated the results by qPCR. We got great results with as little as four times of the cells as prescribed by other CHIP SICAP users.